Thursday, October 10, 2013

Automated Method for Antibiotics Susceptibility

Automated method In the last several years, a variety of instrument-assisted identification and susceptibility test methods have been developed that permit generation of test results in a period of 6—9 hours. as opposed to the 15—24 hour time frame required with traditional overnight method. These newer ‘rapid” methods have, in general, been shown to provide test results nearly as accurate as those derived from traditional overnight tests, d0t the newer tests are more expensive. The clinical impact of this newer technol­ogy and whether it truly facilitates faster and more cost-effective patient care is undergoing clinical study. One study suggests the rapid tests have a positive impact on patient care. The exact role of rapid tests versus traditions’ awaits further clinical experience and comparative studies 4-Antimicrobial concentration gradient methods -The spiral gradient endpoint method employs an agar plate containing a continuous gradient of antibiotic concentration from the center of the plate to the edge; the test organism is applied to the plate in a radial streak, and the MIC is determined by measuring the distance of growth from the edge of the plate -E test E test (AB Biodisk, Solnia. Sweden) is a method based on the diffusion of a continuous concentration gradient of an antimicrobial agent from a plastic strip into an agar medium . This newly developed in Vitro technique was created to overcome several of the disadvantages of the disc diffusion and dilution techniques and also to retain the principle of the agar dilution method by producing on accurate, reproducible, quantitative MIC result

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