Automated Systems In Microbiology

Types of Automated systems used in microbiology

Culture methods
Biochemical identification systems
Antibiotic susceptibility
Antigen/antibody detection
Nucleic acid identification
1-Automated systems for culture

Including :

Bactec 9050
VersaTrek
Bactialert 3D system


Bactec 9050

Using floresent detector.

During growth of bacteria co2 is produced, and begin to diffuse to release hydrogen which enhance emission of florescence detected with special detectors .

Positive samples could be detected within 6 hrs versus 10 days in case of septicemia.
VersaTrek

Depend on consumption/production of multiple gases (co2,H2,O2).

This cause changes in the pressure of the head space which could be detected.

Figure: Our BACTEC System in MUCH
Bactialert 3D system
There is a PH senstive membrane in the bottom of the bottles.

When the organism grow, co2 is released causing changes in PH

This change in PH cause colour change from gray to orange or yellow.

How it works?
Bactialert 3D system
There is a PH senstive membrane in the bottom of the bottles.

When the organism grow, co2 is released causing changes in PH

This change in PH cause colour change from gray to orange or yellow.

How it works?
Culturing techniques for mycobactrium

All techniques depend on liquid media middle brook 7H.

1st Bactec 460

is a radiometeric method using radioactive carbon 14.

The organism utilize carbon to release radioactive co2 into the headspace of the glass vial.

Amount of co2 is proportional to growth (growth index).

Detection within 9-14 days versus 3-6 weeks.

To be continued>>>