The major advantages of PCR are its rapidity and ease of use
as DNA cloning by PCR can be performed in a few hours, using relatively unsophisticated equipment. Sensitivity of PCR is capable of amplifying sequences from minute amounts of target DNA, even the DNA from a single cell Robustness as PCR can permit amplification of specific sequences from material in which the DNA is badly degraded or embedded in a medium from which conventional DNA isolation is problematic (Frey & Suppmann et al ., 1995).
The major disadvantages of PCR are its needing for target DNA sequence information in order to construct specific oligonucleotide primers that permit selective amplification of a particular DNA sequence.Short size and limiting amounts of PCR product are another disadvantages of it. Infidelity of DNA replication as when DNA is replicated in vitro the copying error rates are considerably greater but DNA sequencing of the total PCR products may give the correct sequence (Roux ., 1995).
21 comments:
Am jeremiah, I am testifying about a great hebal man that cured my wife of hepatitis B and liver cirhosis. his name is Dr oniha. My wife was diagnose of hepatities two years ago, i almost spent all i had then, until i saw dr oniha recommendation online, and i call him, then he told me how to get the herb. You can call him on +2347089275769 or email him at dronihaspell@yahoo.com
My name is hoover, my 18 year old daughter, Tricia was diagnosed with herpes 3 years ago. Since then, we have moved from one hospital to another. We tried all kinds of pills, but every effort to get rid of the virus was futile. The bubbles continued to reappear after a few months. My daughter was using 200mg acyclovir pills. 2 tablets every 6 hours and 15g of fusitin cream. and H5 POT. Permanganate with water to be applied twice a day, but all still do not show results. So, I was on the internet a few months ago, to look for other ways to save my only son. Only then did I come across a comment about the herbal treatment of Dr Imoloa and decided to give it a try. I contacted him and he prepared some herbs and sent them, along with guidance on how to use them via the DHL courier service. my daughter used it as directed by dr imoloa and in less than 14 days, my daughter recovered her health. You should contact dr imoloa today directly at his email address for any type of health problem; lupus disease, mouth ulcer, mouth cancer, body pain, fever, hepatitis ABC, syphilis, diarrhea, HIV / AIDS, Huntington's disease, back acne, chronic kidney failure, addison's disease, chronic pain, Crohn's pain, cystic fibrosis, fibromyalgia, inflammatory Bowel disease, fungal nail disease, Lyme disease, Celia disease, Lymphoma, Major depression, Malignant melanoma, Mania, Melorheostosis, Meniere's disease, Mucopolysaccharidosis, Multiple sclerosis, Muscular dystrophy, Rheumatoid arthritis Alzheimer's disease, parkinson's disease, vaginal cancer, epilepsy Anxiety Disorders, Autoimmune Disease, Back Pain, Back Sprain, Bipolar Disorder, Brain Tumor, Malignant, Bruxism, Bulimia, Cervical Disc Disease, Cardiovascular Disease, Neoplasms , chronic respiratory disease, mental and behavioral disorder, Cystic Fibrosis, Hypertension, Diabetes, Asthma, Autoimmune inflammatory media arthritis ed. chronic kidney disease, inflammatory joint disease, impotence, alcohol spectrum feta, dysthymic disorder, eczema, tuberculosis, chronic fatigue syndrome, constipation, inflammatory bowel disease. and many more; contact him at drimolaherbalmademedicine@gmail.com./ also with whatssap- + 2347081986098.
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I'M TOTALLY FREE FROM HERPES VIRUS/HIV/AIDS
HIV/Herpes is a serious and recurring condition that cannot be cured by drugs or injections. But the best way to fight and get rid of the herpes virus permanently is to take natural herbal remedies, I read about DR JAMES, the great herbal Doctor, who cures people of HIV with his powerful herbal mix medicine and spiritual healing. I contacted him to find out how he could help me, and he told me never to be worried that he would help me with the natural herbs' medicine! After 2 days of contacting him, he told me the medicine is ready, and he sent it to me via DHL COURIER SERVICE and it got me in 3 days! I used the medication as he prescribed for me (MORNING and EVENING) and I was cured! It's really like a dream, but I'm so happy! For people suffering from the following diseases Alzheimer disease, Eczema, Shingles, MS, Bulbous Pemphigoid, Diabetes, cancer, PCOS, hypothyroidism, vaginal rashes, Herpes, COPD, HIV, arthritis, HPV, liver disease, autoimmune diseases, Parkinson's disease, Lupus Schizophrenia, Autism, Bipolar disorder, Infertility Get your EX back, Enlargement of Breast, Penis, Butts.CMV, And more should contact him for his herbal medicine, because I am a living witness, and I was cured of herpes virus. And DR James' medicine is legitimate. I sent him what he asked for, and he sent me his medication which I took for 2 weeks and today I am here with a negative result. When I went for the test, I was so happy after I took his herbal mix medicine. CONTACT DR JAMES FOR A PERMANENT CURE Email: DRJAMESHERBALMIX@GMAIL.COM.
He's a good man and he will help you
GODDESS WHO CURE ME OF HERPES WITH HER SPIRITUAL WATER
Hello everyone i want to share a wonderful experience about a GODDESS who cure me of #HERPS with her spiritual water.i still can't believe my eyes about her work without stress or rules abiding the spiritual water.it was a friend of mine who told me her village GODDESS can cure me with her spiritual water i never believe because it does not make sense to me before she further explain that she was chosen by the GODS to serve as a servant,only she alone can go to the river to fetch water for healing their village people.she gave me her email and i contacted her after some time she reply me asking how she can be of help to me and i told her i was diagnosed of herpes over 5years now yet no solution so she ask me to give her some days for consultation behold one[1] week interval she ask me to send her my house address that i will receive the water 4days that all i need to do his to take a shower with the spiritual water morning and evening so i did as she instructed me behold just 4days of using the spiritual water my herpes virus vanish from my body.i never use to believe if such miracles with spiritual things works fast.please get in touch with her for any kinds of virus diseases.
EMAIL ADDRESS [goddessspiritualhealingwater@gmail.com}
PAGE ID {101604095220792}FACEBOOK PAGE {https://www.facebook.com/Queen-Goddess-101604095220792/
}
After years of working in construction and smoking cigarettes,I was diagnosed with COPD and pulmonary fibrosis. As my illness progressed, I found myself relying on supplemental oxygen around the clock. Even getting out of bed became a challenge.My doctors and specialists recommended a lung transplant, but after researching the pros and cons of such a drastic procedure, I decided to try something less invasive. I contacted multivitamin herbal cure cape town for organic natural treatment you can search for them on google. Following the COPD and pulmonary fibrosis herbal treatment procedure , my lung function has improved dramatically after my first four weeks of the herbal formula .My quality of life has gotten a lot better. I was amazed that I could breathe without any dead air. I no longer need oxygen and look forward to continued improvement because a specialist told me already I have a 97% chance of getting my condition totally due to the herbal treatment effectiveness . If you or someone you love has COPD, pulmonary fibrosis or another chronic lung disease, and would like to see improvements like mine they even guarantee me totally cure once the treatment is done , their website is www.multivitamincare.org Call (or SMS) them : +1 (786) 544-5148 they will put you through on the herbal process .
My name is James Isabella,I am from the USA . It’s a pleasure for me to write this testimony about how I got my Genital Herpes cured a month ago. I have been reading so many comments of some people who were cured from various diseases by Dr Biya, but I never believed them. I was hurt and depressed so I was too curious and wanted to try Dr Biya, then i contacted him through his email when i contacted him, he assured me 100% that he will help me, i pleaded with him to help me out. My treatment was a great success, he healed me just as he promised. He sent me his medication and asked me to go for check up after 3 weeks of taking the medication. i agreed with him i took this medication and went for check up after a month later , to my greatest surprise my result was negative after the treatment, i am really happy that i am cured and healthy again. I have waited for 3weeks to be very sure I was completely healed before writing this testimony. I did another blood test one week ago and it was still Herpes negative. So I guess it’s time I recommend anyone going through Herpes contact him through What’s app +2348111856507 or Gmail: drbiyajatto@gmail.com God bless you Dr Biya. Dr Biya also has remedy to other diseases like COLD SORES,HIV/AIDS,DIABETES.CANCER,HIGH BLOOD PRESSURE,LUPUS,FIBROIDS AND MANY MORE. https://drbiyajatto.wixsite.com/drbiyajattoherbalhom https://m.youtube.com/results?search_query=dr+biya+herbal+medication
I experienced dark urine and ribs pain, so I went to the hospital for treatment and I was diagnosed for chronic HEPATITIS B. The doctor gave me drugs to slowdown the viral load which later worsens the ailment because the drugs weren’t effective anymore. And I was so scared because they say it has no cure, I told a friend about it and luckily he told me he ones battled same problem in the past but he was saved by Dr. Iyabiye. He gave me his contact and I reached him and I became well and tested negative after the treatment. Here is his contact if you have such case. Contact: +234-815-857-7300, (iyabiyehealinghome@gmail.com)
HOW I GOT CURED FROM HPV WARTS Greetings to the general public, i want to tell about how i was cured of HPV disease by a Doctor called DR MOMOH i was browsing through the Internet searching for remedy on HPV and i saw comment of people talking about how DR MOMOH cured them. I Was scared because i never believed in the Internet but i was convince to give him a try because i having no hope of been cured of HPV so i decided to contact him with his email that was listed on the comment when i contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without problem, my HPV result came out negative. I pray for you God will give you everlasting life, you shall not die before your time for being a sincere and great man. Am so happy, you can also contact him if you have any problem Email: dr.momoh9@gmail.com website on: https://drmomohherbaltreatment.wordpress.com or whatsApp him on this number +2347083724098 1. LOVE SPELL 2. ALS CURE 3. HEPATITIS B 4. HIV/AIDS CURE 5. HERPES CURE 6 SPIRITUAL HEALERS
I am here to give my testimony about Dr Ubarlo who helped me.. i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by Dr Ubarlo, i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Dr Ubarlo cured them. when i contacted him he gave me hope and send a Herbal medicine to me that i took for just 2 weeks and it seriously worked for me, my HERPES result came out negative. I am so happy as i am sharing this testimony. My advice to you all who thinks that there is no cure for herpes that is Not true just contact him and get cure from Dr Ubarlo healing herbal cure of all kinds of sickness you may have like
(1) CANCER,
(2) DIABETES,
(3) HIV&AIDS,
(4) URINARY TRACT INFECTION,
(5) CANCER,
(6) IMPOTENCE,
(7) BARENESS/INFERTILITY
(8) DIARRHEA
(9) ASTHMA
(10)SIMPLEX HERPES AND GENITAL
(11)COLD SORE
(12) ENLARGEMENT CREAM
and mare that are not mentioned here
. he also cure my friend from cervical cancer. so contact him thruogh his Email address drubarlohome@gmail.com or whatsapp him on +2348119508814
it"s obvious some patients with Herpes Virus are being enslaved to the antiviral and other supplementary Orthodox medicine just to help suppress the virus and not a cure. I have been with the virus since 2015 until I was introduced by a blogger who also narrated her story online on how she was cured of HPV after using Dr ALUDA Herbal Medicine. This is a year and 2 weeks since I was delivered from Herpes VIRUS. All thanks to God for using this Great herbalist to heal me. I have promised to keep telling good things about Dr ALUDA. Please feel free to share Your problems with him and don’t forget to tell him I did refer you to Him. Thanks. Write him on whatsapp +2348145636867 or mail him Draludaharbalhome @gmail. com He deals with Alzheimer virus, Cancer, HIV, Herpes, Genital, warts, ALS, HBV, UTI, Virginal infection, Genital, Wart, HPV, Hepatitis A/B, Good luck, HSV, Cold Sores, Diabetes 1 & 2, Pregnancy, Ex back.
ALL THANKS TO DR OSO WITH HIS HERBAL PORTION I WAS COMPLETELY CURED FROM BREAST CANCER. I'm here again to appreciate it. DR OSO God will always continue to bless you more abundantly, for the good works you do in my life, I will always keep on writing good and posting my testimonies about you on the Internet, I’m MARY JOHNSON from TEXAS . I was tested breast cancer positive, I saw a blog on how DR OSO cured people with his herbal portion, i did not believe in natural medicine but i just decided to give him a try, I contacted him, and explain my situation to him, few day later he sent me the herb, after taking DR OSO herbal medicine for few weeks i went to hospital for check up so luckily i was healed with his herbal portion, i am so happy. If you have any type of cancer problem or you are also infected with any kind of disease,
contact him drosohaberhome@gmail.com
call/WhatsApp +2348162084839
for advice and for his product, I hope this testimony also helps someone out there .. Here is the dr website.....
https://sites.google.com/view/drosohaberhome/
His blog page
https://drosohaberhome.blogspot.com
you can also email me for more info about DR oso via maryjohnson9700@gmail.com ALL THANK TO DR OSO .
I started on COPD Herbal treatment from Ultimate Life Clinic, the treatment worked incredibly for my lungs condition. I used the herbal treatment for almost 4 months, it reversed my COPD. My severe shortness of breath, dry cough, chest tightness gradually disappeared. Reach Ultimate Life Clinic via their website www.ultimatelifeclinic.com . I can breath much better and It feels comfortable!
HERPES INFECTION CURE IS 100% AVAILABLE WITH THE HELP OF DR OGBERAESE HERBAL MEDICINE.Joy and happiness is all i can see around ever since i came in contact with this great man Called Dr Ogberaese. i complained bitterly to him about me having herpes only for him to tell me it’s a minor stuff. He told me he has cured thousands of people but i did not believe until he sent me the herbal medicine and i took it as instructed by this great man, only to go to the hospital after two weeks for another test and i was confirmed negative. For the first time in four years i was getting that result. i want to use this medium to thank this great man. His name is Dr Ogberaese , i came in with his email through a friend in USA called Edith Rose and ever since then my live has been full with laughter and great peace of mind. i urge you all with herpes or HSV1&2 to contact him if you are willing to give him a chance. you can contact him through his email drogberaese54@gmail.com or you can also WhatsApp him +2348073818653
Very informative, because it compresses most of the information needed and I already have known that, the best solution to Paralysis is herbal medicines. My family and I are proof to this including Rex my friend.
I came to trust Nze Njoku Herbal Home after many months that, they have healed my friend from Pain and Arthritis without any aftereffect.
So I also contacted Nze Njoku for herbal treatment for my father who have suffered paralysis for 6years.
My father took this herbal medicine from Nze Njoku Herbal Home without any lifestyle change which healed him of paralysis within 4weeks.
As I write this my father is doing great on his feet and most importantly he has started his business effectively as if nothing happened to him.
Feel free to contact him on;
nzenjokuherbalhome@gmail.com
Big thanks to Dr. Iyabiye who treated/cure my brother of chronic HEPATITIS B and Liver Cirrhosis. My brother was diagnosed of the disease last year and will try several antiviral medications but they were all to no avail. I went online out of frustration for a new medication and I came across this doctor’s recommendation. I reached out to him and my brother got cured after the completion of the medicine. Dr. iyabiye’s contact: +234-815-857-7300 and email: iyabiyehealinghome@gmail.com
My name is Thomas White, from United states, i have been Herpes positive and it is just like been through hell; well special thanks to God almighty for using Dr Twaha, the greatest Herbal doctor in curing my herpes disease. I was diagnosed of this disease in the year 2004. Although I was taking my medications I was not myself. Until a few weeks ago. My friend came to me and told me that he saw many testimonies on how Dr. Twaha cured people Herpes disease. Although I never believe in herbs, I said to myself "seeing is believing" I took his name and searched it on GOOGLE, I saw many testimonies myself. Quickly, I copied his email: drlregbeyen10000@gmail.com I emailed him, he gave me a form to fill which I did, then he sent me a message and told me that he required some items in which he will use in preparing the cure for me and he told me that it will cost me some money which i sent to him because I need the cure urgent, He bought the items and prepared a cure for me, then he sent the parcel to me through courier delivery service. I paid the courier services, I got my parcel, it was a bottle of ointment as described by Dr,Twaha After taking the ointment he told me to do, the herpes test and the result came out negative. Quickly I messaged him and told him what happened he congratulated me. I promised to tell the world about him. You can contact him with any problem via email: ( drlregbeyen10000@gmail.com ) or contact him on WhatsApp +2349038518881 thank you Dr Twaha
Do not let your Doctor lie to you and make you believe there is no cure for Herpes, I was a herpes patient until I came in contact with Dr OSO who used his natural herbs to cure me of Lungs cancer now I'm negative to genital email him; drosohaberhome@gmail.com
- [ ] I'm here to testify about the great work Dr Osebor did for me. I have been suffering from (HERPES) disease for the past 5 years and had constant pain, especially in my knees. During the first year,I had faith in God that i would be healed someday.This disease started circulating all over my body and i have been taking treatment from my doctor, few weeks ago I came across a testimony of one lady on the internet testifying about a Man called Dr Osebor on how he cured her from HIV Virus. And she also gave the email address of this man and advise anybody to contact Dr Osebor for help for any kind of sickness that he would be of help, so I emailed him on ( oseborwinbacktemple@gmail.com ) telling him about my (HERPES Virus) he told me not to worry that i was going to be cured!! Well i never believed it,, well after all the procedures and remedy given to me by this man few weeks later i started experiencing changes all over me as Dr Osebor assured me that i will be cured,after some time i went to my doctor to confirmed if i have be finally healed behold it was TRUE, So
- [ ] friends my advise is if you have such sickness or any other at all you can contact Dr Osebor via email. { oseborwinbacktemple@gmail.com }or call or what sapp him on( +2348073245515 )
- [ ] DR osebor CAN AS WELL CURE THE FOLLOWING DISEASE:-
- [ ] HIV/AIDS
- [ ] HERPES
- [ ] CANCER
- [ ] ALS
- [ ] cancer
- [ ] Diabetes
eye problem etc.
I want to say a very big thank you to Doctor Patrick for helping with natural cure medicines for epilepsy, I used the medicines for one month only and I was cured totally from epilepsy and its over eight months now, I haven't experienced or had any seizure, I want to say thank you once more again Doctor Patrick for what you have done for me, his email contact is herbalmedicalhome@gmail.com +2348021312463
Hello everyone i was diagnosed of herpes simplex virus for about 8 months ,I’ve lost all hope and my doctor said there is no possible cure and I have been taken antiviral to sustain myself I have had several out break from the time I was diagnosed till I got to know about Dr.Ebhota through a friend who he cured of Herpes . I contacted him and he sent me his herbal remedy which I took as he directed me to do for some days and I went to the hospital after consuming his Herbal medicine and I was confirmed Herpes Negative after 8 months of pains and depression , it was indeed a miracle, Dr.ebhota is a great man, I have heard so much about how he has help lot of people, He also cure ALS, HEPATITIS B, CANCER, HERPES, DIABETES and lots more. If you have any issues you can contact him for help add him on whatsapp +2348089535482. or via email:Email drebhotasolution@gmail.com
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